Biodiversity of Saccharomyces cerevisiae Yeasts in Spontaneous Alcoholic Fermentations: Typical Cellar or Zone Strains?

  1. Santamaría, Pilar 1
  2. López, Rosa 1
  3. del Patrocinio Garijo, Maria 1
  4. Escribano, Rocío 1
  5. González-Arenzana, Lucía 1
  6. López-Alfaro, Isabel 1
  7. Rosa Gutiérrez, Ana 1
  1. 1 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

Buch:
Advances in Grape and Wine Biotechnology

ISBN: 9781789846126

Datum der Publikation: 2019

Art: Buch-Kapitel

DOI: 10.5772/INTECHOPEN.84870 GOOGLE SCHOLAR lock_openOpen Access editor

Zusammenfassung

Spontaneous fermentation is the most traditional way and a low-intervention method for conducting alcoholic fermentation in wineries, giving rise to the most complex wine profiles. However, inoculation with single culture inocula of Saccharomyces cerevisiae strains has become widespread in the modern wine industry. Nevertheless, some authors have pointed out that the use of the same yeasts in all the winegrowing regions of the world can cause a loss of typicity and have a standardizing effect on the wines. For this reason, many wineries and regions are carrying out programs of isolation and selection of yeasts that are typical of their vineyards/wineries. The aim of this work was to study the ecology of spontaneous fermentations in 11 wineries from all over the Rioja qualified designation of origin (Spain) during 3–4 consecutive years in order to establish the existence of typical strains belonging to wineries, sub-zones, or regional ecosystems. The results obtained showed a great diversity of strains of Saccharomyces cerevisiae in each fermentation studied. These strains were different each year in each winery, and hardly any common strains were detected between neighboring wineries, which would indicate that there are no representative strains from the winery or the area.

Bibliographische Referenzen

  • Varela C, Siebert T, Cozzolino D, Rose L, McLean H, Henschke PA. Discovering a chemical basis for differentiating wines made by fermentation with wild indigenous and inoculated yeasts: Role of yeasts volatile compounds. Australian Journal of Grape and Wine Research. 2009;15:238-248. DOI: 10/1111/j.1755-0238.2009.00054.x
  • Santamaría P, López R, López E, Garijo P, Gutiérrez AR. Permanence of yeast inocula in the winery ecosystem and presence in spontaneous fermentations. European Food Research and Technology. 2008;227(5):1563-1567. DOI: 10.1007/s00217-008-0855-5
  • Gutiérrez AR, Santamaría P, Epifanio S, Garijo P, López R. Ecology of spontaneous fermentation in one winery during five consecutive years. Letters in Applied Microbiology. 1999;29:411-415. DOI: 10.1046/j.1472-765X.1999.00657.x
  • Vezinhet F, Hallet JN, Valade M, Poulard A. Ecological survey of wine strains by molecular methods of identification. American Journal of Enology and Viticulture. 1992;43:83-86
  • Blanco P, Ramilo A, Cerdeira M, Orriols I. Genetic diversity of wine Saccharomyces cerevisiae strains in an experimental winery from Galicia (NW Spain). Antonie Van Leeuwenhoek. 2006;89:351-357. DOI: 10.1007/s10482-005-9038-6
  • Torija M, Rozès N, Poblet M, Guillamón JM, Mas A. Yeast population dynamics in spontaneous fermentations: Comparison between two different wine-producing areas over a period of three years. Antonie Van Leeuwenhoek. 2001;79:345-352. DOI: 10.1023/A:1012027718701
  • Knight S, Klaere S, Fedrizzi B, Goddard MR. Regional microbial signatures positively correlate with differential wine phenotypes: Evidence for a microbial aspect to terroir. Scientific Reports. 2015;5:14233. DOI: 10.1038/srep14233
  • Capece A, Granchi L, Guerrini S, Mangani S, Romaniello R, Vincenzini M, et al. Diversity of Saccharomyces cerevisiae strains isolated from two italian wine-producing regions. Frontiers in Microbiology. 2016;7:1-11. DOI: 10/3389/fmicb.2016.01018
  • Tristezza M, Fantastico L, Vetrano C, Bleve G, Corallo D, Grieco F, et al. Molecular and technological characterization of Saccharomyces cerevisiae strains isolated from natural fermentation of Susumaniello grape must in Apulia, southern Italy. International Journal of Microbiology. 2014:897428. DOI: 10.1155/2014/897428
  • Schvarczová E, Stefaniková J, Jankura E, Kolek E. Selection of autochthonous Saccharomyces cerevisiae strains for production of typical Pinot gris wines. Journal of Food and Nutrition Research. 2017;56(4):389-397
  • Orlic S, Vojvoda T, Babic KH, Arroyo-López FN, Jeromel A, Kocina B, et al. Diversity and oenological characterization of indigenous Saccharomyces cerevisiae associated with Zilavka grapes. World Journal of Microbiology and Biotechnology. 2010;26:1483-1489. DOI: 10.1007/s11274-010-0323-9
  • Querol A, Barrio E. A rapid and simple method for the preparation of yeast mitocondrial DNA. Nucleic Acids Research. 1990;18:1657
  • López I. Detección y control por técnicas de la Biología Molecular de bacterias lácticas autóctonas responsables de la fermentación maloláctica en vinos de la D.O.Ca. Rioja [thesis]. University of La Rioja; 2004
  • Clemente-Jiménez JM, Mingorance-Cazorla L, Martínez-Rodríguez S, Las Heras-Vázquez FJ, Rodríguez-Vico F. Molecular characterization and oenological properties of wine yeasts isolated during spontaneous fermentation of six varieties of grape must. Food Microbiology. 2004;21:149-155. DOI: 10.1016S0740-0020(03)00063-7
  • Santamaría P, Garijo P, López R, Tenorio C, Gutiérrez AR. Analysis of yeast population during spontaneous alcoholic fermentation: Effect of the age of the cellar and the practice of inoculation. International Journal of Food Microbiology. 2005;103:49-56. DOI: 10.1016/j.ijfoodmicro.2004.11.024
  • Jemec KP, Cadez N, Zagorc T, Vuvic V. Yeast population dynamics in five spontaneous fermentations of Malvasia musts. Food Microbiology. 2001;18(3):247-259. DOI: 10.1016/fmic.2001.0396
  • Sangorrín M, Zajonskovsky I, van Broock M, Caballero A. The use of killer biotyping in an ecological survey of yeast in an old Patagonian winery. World Journal of Microbiology and Biotechnology. 2002;18(2):115-120. DOI: 10.1023/A:101441722 2890
  • Versavaud A, Courcoux P, Roulland C, Dulau L, Hallet JN. Genetic diversity and geographical distribution of wild Saccharomyces cerevisiae strains from the wine-producing area of Charentes, France. Applied Environmental Microbiology. 1995;61(10):3521-3529
  • Schütz M, Gafner J. Dynamics of the yeast strain population during spontaneous alcoholic fermentation determined by CHEF gel electrophoresis. Letters in Applied Microbiology. 1994;19(4):253-257. DOI: 10.1111/j.1472-765X.1994.tb00957.x
  • Granchi L, Ganucci D, Viti C, Giovannetti L, Vincenzini M. Saccharomyces cerevisiae biodiversity in spontaneous commercial fermentations of grape musts with adequate and inadequate assimilable-nitrogen content. Letters in Applied Microbiology. 2003;36:54-58. DOI: 10.1046/j.1472-765X.2003.01263.x
  • Garijo P. Estudio del aire como vía de diseminación de microorganismos enológicos [thesis]. University of La Rioja; 2013
  • Van der Westhuizen TJ, Augustyn OPH, Pretorius IS. Geographical distribution of indigenous Saccharomyces cerevisiae strains isolated from vineyard in the Coastal Regions of the Western Cape in South Africa. South African Journal of Enology and Viticulture. 2000;21(1):3-9. DOI: 10.21548/21-1-2179
  • Fleet GH. Yeast interactions and wine flavour. International Journal of Food Microbiology. 2003;86(1-2):11-22. DOI: 10.1016/S0168-1605(03)00245-9
  • Ocón E, Garijo P, López I, Santamaría P, Gutiérrez AR, Tenorio C, et al. Quantitative and qualitative analysis of non-Saccharomyces yeasts in spontaneous alcoholic fermentations. European Food Research and Technology. 2010;230(6):885-891. DOI: 10.1007/s00217-010-1233-7
  • Le Jeune C, Claude E, Demuyter C, Lollier M. Evolution of the population of Saccharomyces cerevisiae from grape to wine in a spontaneous fermentation. Food Microbiology. 2006;23:709-716. DOI: 10.1016/j.fm.2006.02.007