Genomic diversity of Oenococcus oeni populations from Castilla La Mancha and La Rioja Tempranillo red wines

  1. González-Arenzana, L. 2
  2. Pérez-Martín, F. 1
  3. Palop, M.L. 1
  4. Seseña, S. 1
  5. Santamaría, P. 2
  6. López, R. 2
  7. López-Alfaro, I. 2
  1. 1 Universidad de Castilla-La Mancha
    info

    Universidad de Castilla-La Mancha

    Ciudad Real, España

    ROR https://ror.org/05r78ng12

  2. 2 Instituto de Ciencias de la Vid y del Vino
    info

    Instituto de Ciencias de la Vid y del Vino

    Logroño, España

    ROR https://ror.org/01rm2sw78

Revista:
Food Microbiology

ISSN: 0740-0020

Any de publicació: 2015

Volum: 49

Pàgines: 82-94

Tipus: Article

DOI: 10.1016/J.FM.2015.02.001 SCOPUS: 2-s2.0-84923076269 WoS: WOS:000352202900010 GOOGLE SCHOLAR

Altres publicacions en: Food Microbiology

Resum

The Oenococcus oeni populations of Tempranillo wines from Castilla La Mancha and La Rioja winemaking regions were analysed from one to three years and up to ten wineries. The objective was to evaluate the genetic variability and the O.oeni population structure. For this purpose a MLST scheme based on four loci (. gyrB, purK, pgm and recP genes) and PFGE with SfiI restriction enzyme were developed for later combination. The results showed an O.oeni population completely adapted to winemaking regions. Apurifying selection influenced the genes evolution, especially recP that along with purK were the most interesting loci to analyse the genetic variability of the isolates. In this way linkage disequilibrium and intergenic and intragenic recombination were determined between isolates. PFGE typing with UPGMA data were not coincident with the phylograms assessed for MLST by Maximum likelihood and combination of both techniques differentiated all the isolates as strains. Those results led the research to conclude that O.oeni population from CM and LR was a panmictic population with a slight clonal evolution, so subpopulations could not be described. A broader study including more winemaking regions with different grape varieties and distinct ways of elaborating would be interesting to complete the knowledge about O.oeni populations. © 2015 Elsevier Ltd.