Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

  1. Á.G. Solaesa 1
  2. M.T. Sanz 1
  3. R. Melgosa 1
  4. S.L. Bucio 1
  5. S. Beltrán 1
  1. 1 University of Burgos, Spain
Journal:
Grasas y aceites

ISSN: 0017-3495,  1988-4214

Year of publication: 2015

Volume: 66

Issue: 4

Type: Article

DOI: 10.3989/GYA.0238151 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Grasas y aceites

Sustainable development goals

Abstract

The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%).

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Funding information

Funders

  • Ministerio de Economía y Competitividad
    • BES-2013-063937

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