Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

  1. Á.G. Solaesa 1
  2. M.T. Sanz 1
  3. R. Melgosa 1
  4. S.L. Bucio 1
  5. S. Beltrán 1
  1. 1 University of Burgos, Spain
Journal:
Grasas y aceites

ISSN: 0017-3495,  1988-4214

Year of publication: 2015

Volume: 66

Issue: 4

Type: Article

DOI: 10.3989/GYA.0238151 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Grasas y aceites

Sustainable development goals

Abstract

En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI), mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en peso de AGPI y un bajo contenido en ácidos grasos libres (menor del 18 % en peso).

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Funding information

Funders

  • Ministerio de Economía y Competitividad
    • BES-2013-063937

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